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Education and Experience:
B.S., Department of Chemical Engineering, College of Engineering and Science, Feng Chia University 1996-2000
M.S., Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University 2003-2005
Ph.D., Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University 2005-2012
Visiting Scholar, Department of Ophthalmology, University of Cincinnati 2008
Postdoctoral Fellow, Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University (2012-2013, 2016-2020, 2023-2024)
Postdoctoral Scholar, Department of Medicine, University of Chicago 2013-2014
Project Assistant Research Fellow, International Center for Wound Repair and Regeneration, National Cheng Kung University 2020-2023
Assistant Research Scholar, Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University 2024-2025
Assistant Professor, Department of Physiology, College of Medicine, National Cheng Kung University 2025-
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- Chung-Sheng Shi, Guey-Yueh Shi, Yi-Sheng Chang, Huai-Song Han,Cheng-Hsiang Kuo, Che Liu, Huey-Chun Huang, Yu-Jia Chang, Pin-Shern Chen, Hua-Lin Wu*. 2005 Apr. Evidence of human thrombomodulin domain as a novel angiogenic factor. Circulation. 5;111(13):1627-36.
- Chung-Sheng Shi, Huey-Chun Huang, Hua-Lin Wu, Cheng-Hsiang Kuo, Bi-Ing Chang, Ming-Shi Shiao, Guey-Yueh Shi*. 2006 Jul. Salvianolic acid B modulates hemostasis properties of human umbilical vein endothelial cells. Thromb Res.;119(6):769-75.
- Hua-Lin Wu, Chi-Iou Lin, Yuan-Li Huang, Pin-Shern Chen, Cheng-Hsiang Kuo, Mei-Shing Chen, Georgiana Cho-Chen Wu, Guey-Yueh Shi, Hsi-Yuan Yang, Hsinyu Lee *. 2008 Feb. Lysophosphatidic acid stimulates thrombomodulin lectin-like domain shedding in human endothelial cells. Biochem Biophys Res Commun. 29;367(1):162-8.
- Chung-Sheng Shi, Guey-Yueh Shi, Hsi-Min Hsiao, Yuan-Chung Kao, Kuan-Lin Kuo, Chih-Yuan Ma, Cheng-Hsiang Kuo, Bi-Ing Chang, Chuan-Fa Chang, Chun-Hung Lin, Chi-Huey Wong, Hua-Lin Wu*. 2008 Nov. Lectin-like domain of thrombomodulin binds to its specific ligand Lewis Y antigen and neutralizes lipopolysaccharide-induced inflammatory response. Blood. 112(9):3661-70.
- Hsi-Ju Wei, Yi-Heng Li, Guey-Yueh Shi, Shu-Lin Liu, Po-Chiao Chang, Cheng-Hsiang Kuo, Hua-Lin Wu *. 2011 Nov. Thrombomodulin domains attenuate atherosclerosis by inhibiting thrombin-induced endothelial cell activation. Cardiovasc Res. 92(2):317-27.
- Cheng-Hsiang Kuo, Po-Ku Chen, Bi-Ing Chang, Meng-Chen Sung, Chung-Sheng Shi, Jeng-Shin Lee, Chuan-Fa Chang, Guey-Yueh Shi*, Hua-Lin Wu*. 2012 Feb. The recombinant lectin-like domain of thrombomodulin inhibits angiogenesis through interaction with Lewis Y antigen. Blood. 119(5):1302-13.
- Yi-Heng Li, Cheng-Hsiang Kuo, Guey-Yueh Shi, Hua-Lin Wu*. The role of thrombomodulin lectin-like domain in inflammation. 2012 Mar.
- Yun-Yan Hsu, Guey-Yueh Shi, Cheng-Hsiang Kuo, Shu-Lin Liu, Ching-Ming Wu, Chih-Yuan Ma, Feng-Yi Lin, Hsi-Yuan Yang, Hua-Lin Wu*. 2012 Aug. Thrombomodulin is an ezrin-interacting protein that controls epithelial morphology and promotes collective cell migration. FASEB J.;26(8):3440-52.
- Tsung-Lin Cheng, Yu-Ting Wu, Chao-Han Lai, Yuan-Chung Kao, Cheng-Hsiang Kuo, Shu-Lin Liu, Yun-Yan Hsu, Po-Ku Chen, Chia-Fong Cho, Kuan-Chieh Wang, Wei-Ling Lin, Bi-Ing Chang, Chun-Ming Chen, Hartmut Weiler, Guey-Yueh Shi, Hua-Lin Wu *. 2013 Jun. Thrombomodulin regulates keratinocyte differentiation and promotes wound healing. J Invest Dermatol. 133(6):1638-45.
- Po-Ku Chen, Bi-Ing Chang, Cheng-Hsiang Kuo, Pin-Shern Chen, Chia-Fong Cho, Chuan-Fa Chang, Guey-Yueh Shi, Hua-Lin Wu*. 2013 Nov. Thrombomodulin functions as a plasminogen receptor to modulate angiogenesis. FASEB J. 27(11):4520-31.
- Chao-Han Lai, Guey-Yueh Shi, Fang-Tzu Lee, Cheng-Hsiang Kuo, Tsung-Lin Cheng, Bi-Ing Chang, Chih-Yuan Ma, Fu-Chih Hsu, Yu-Jen Yang, Hua-Lin Wu*. 2013 Dec. Recombinant human thrombomodulin suppresses experimental abdominal aortic aneurysms induced by calcium chloride in mice. Ann Surg. 258(6):1103-10.
- Shun-Min Yang, Shuk-Man Ka, Hua-Lin Wu, Yu-Chuan Yeh, Cheng-Hsiang Kuo, Kuo-Feng Hua, Guey-Yueh Shi, Yi-Jen Hung, Fone-Ching Hsiao, Sung-Sen Yang, Yi-Shing Shieh, Shih-Hua Lin, Chyou-Wei Wei, Jeng-Shin Lee, Chu-Yi Yang, Ann Chen*. 2014 Feb. Thrombomodulin domain 1 ameliorates diabetic nephropathy in mice via anti-NF-κB/NLRP3 inflammasome-mediated inflammation, enhancement of NRF2 antioxidant activity and inhibition of apoptosis. Diabetologia. 57(2):424-34.
- Ting-Hsing Chao, Wei-Chuan Tsai, Ju-Yi Chen, Ping-Yen Liu, Hsing-Chun Chung, Shi-Ya Tseng, Cheng-Hsiang Kuo, Guey-Yueh Shi, Hua-Lin Wu, Yi-Heng Li*. 2014 Mar. Soluble thrombomodulin is a paracrine anti-apoptotic factor for vascular endothelial protection. Int J Cardiol. 172(2):340-9.
- Cheng-Hsiang Kuo#, Lorraine Leon#, Eun Ji Chung, Ru-Ting Huang, Timothy J Sontag, Catherine A Reardon, Godfrey S Getz, Matthew Tirrell*, Yun Fang*. 2014 Dec. Inhibition of atherosclerosis-promoting microRNAs via targeted polyelectrolyte complex micelles. J Mater Chem B. 2(46):8142-8153.
- Yi-Hsun Huang, Ching-Chang I, Cheng-Hsiang Kuo, Yun-Yan Hsu, Fang-Tzu Lee, Guey-Yueh Shi, Sung-Huei Tseng, Hua-Lin Wu*. 2015 Mar. Thrombomodulin promotes corneal epithelial wound healing. PLoS One. 10(3):e0122491.
- Cheng-Hsiang Kuo, Meng-Chen Sung, Po-Ku Chen, Bi-Ing Chang, Fang-Tzu Lee, Chia-Fong Cho, Tai-Tzu Hsieh, Yu-Ching Huang, Yi-Heng Li, Guey-Yueh Shi, Chwan-Yau Luo, Hua-Lin Wu*. 2015 Jan. FGFR1 mediates recombinant thrombomodulin domain-induced angiogenesis. Cardiovasc Res. 105(1):107-17.
- Cheng-Hsiang Kuo, Bi-Ing Chang, Fang-Tzu Lee, Po-Ku Chen, Jeng-Shin Lee, Guey-Yueh Shi, Hua-Lin Wu*. 2015 Sep. Development of recombinant AAV2/8 carrying kringle domains of human plasminogen for sustained expression and cancer therapy. Hum Gene Ther. 26(9):603-13.
- Congqing Wu, Ru-Ting Huang, Cheng-Hsiang Kuo, Sandeep Kumar, Chan Woo Kim, Yen-Chen Lin, Yen-Ju Chen, Anna Birukova, Konstantin G Birukov, Nickolai O Dulin, Mete Civelek, Aldons J Lusis, Xavier Loyer, Alain Tedgui, Guohao Dai, Hanjoong Jo, Yun Fang*. 2015 Jul. Mechano-sensitive PPAP2B regulates endothelial responses to athero-relevant hemodynamic forces. Circ Res. 117(4):e41-53.
- Chao-Han Lai, Kuan-Chieh Wang, Cheng-Hsiang Kuo, Fang-Tzu Lee, Tsung-Lin Cheng, Bi-Ing Chang, Yu-Jen Yang, Guey-Yueh Shi, Hua-Lin Wu*. 2017 Mar. Recombinant adeno-associated virus vector carrying the thrombomodulin lectin-like domain for the treatment of abdominal aortic aneurysm.
- David Wu, Ru-Ting Huang, Robert B Hamanaka, Matt Krause, Myung-Jin Oh, Cheng-Hsiang Kuo, Recep Nigdelioglu, Angelo Y Meliton, Leah Witt, Guohao Dai, Mete Civelek, Nanduri R Prabhakar, Yun Fang, Gökhan M Mutlu* 2017 May. HIF-1α is required for disturbed flow-induced metabolic reprogramming in human and porcine vascular endothelium. eLife. 6. pii: e25217.
- Christopher Poon, Sampreeti Chowdhuri, Cheng-Hsiang Kuo, Yun Fang, Francis J Alenghat, Danielle Hyatt, Kian Kani, Mitchell E Gross, Eun Ji Chung*. 2017 Dec. Protein mimetic and anticancer properties of monocyte-targeting peptide amphiphile micelles. ACS Biomater. Sci. Eng. 3(12):3273-3282.
- Julia J Mack, Thiago S Mosqueiro, Brian J Archer, William M Jones, Hannah Sunshine, Guido C Faas, Anais Briot, Raquel L Aragón, Trent Su, Milagros C Romay, Austin I McDonald, Cheng-Hsiang Kuo, Carlos O Lizama, Timothy F Lane, Ann C Zovein, Yun Fang, Elizabeth J Tarling, Thomas Q de Aguiar Vallim, Mohamad Navab, Alan M Fogelman, Louis S Bouchard, M Luisa Iruela-Arispe*. 2017 Nov. NOTCH1 is a mechanosensor in adult arteries. Nat Commun. 8(1):1620.
- Tsung-Lin Cheng, Po-Ku Chen, Wei-Kai Huang, Cheng-Hsiang Kuo, Chia-Fong Cho, Kuan-Chieh Wang, Guey-Yueh Shi, Hua-Lin Wu, Chao-Han Lai*. 2018 Dec. Plasminogen/thrombomodulin signaling enhances VEGF expression to promote cutaneous wound healing. J Mol Med (Berl). 96(12):1333-1344.
- Yi-Kai Hong, Yao-Chou Lee, Tsung-Lin Cheng, Chao-Han Lai, Chao-Kai Hsu, Cheng-Hsiang Kuo, Yun-Yan Hsu, Jui-Ting Li, Bi-Ing Chang, Chih-Yuan Ma, Shu-Wha Lin, Kuan-Chieh Wang, Guey-Yueh Shi, Hua-Lin Wu*. 2019 Oct. Tumor endothelial marker 1 (TEM1/Endosialin/CD248) enhances wound healing by interacting with platelet-derived growth factor receptors. J Invest Dermatol. 139(10):2204-2214.
- Gang-Hui Lee, Chia-Lin Chang, Wen-Tai Chiu, Tsun-Hsien Hsiao, Po-Yuan Chen, Kuan-Chieh Wang, Cheng-Hsiang Kuo, Bing-Hung Chen, Guey-Yueh Shi, Hua-Lin Wu*, Tzu-Fun Fu*. 2019 Aug. A thrombomodulin-like gene is crucial to the collective migration of epibolic blastomeres during germ layer formation and organogenesis in zebrafish. J Biomed Sci. 26(1):60.
- Chao-Han Lai, Ching-Wen Chang, Fang-Tzu Lee, Cheng-Hsiang Kuo, Jong-Hau Hsu, Chung-Pin Liu, Hua-Lin Wu, Jwu-Lai Yeh*. 2020 Mar. Targeting vascular smooth muscle cell dysfunction with xanthine derivative KMUP-3 inhibits abdominal aortic aneurysm in mice. Atherosclerosis. 297:16-24.
- Chao-Han Lai, Ching-Wen Chang, Fang-Tzu Lee, Cheng-Hsiang Kuo, Jong-Hau Hsu, Chung-Pin Liu, Hua-Lin Wu, Jwu-Lai Yeh*. 2020 Apr. Data supporting the effects of xanthine derivative KMUP-3 on vascular smooth muscle cell calcification and abdominal aortic aneurysm in mice. Data Brief. 30:105550.
- Cheng-Hsiang Kuo*, Yi-Hsun Huang, Po-Ku Chen, Gang-Hui Lee, Ming-Jer Tang, Edward M Conway, Guey-Yueh Shi, Hua-Lin Wu*. 2021 May. VEGF-Induced endothelial podosomes via ROCK2-dependent thrombomodulin expression initiate sprouting angiogenesis. Arterioscler Thromb Vasc Biol. 41(5):1657-1671.
- Lan-Yun Chang, Chao-Han Lai, Cheng-Hsiang Kuo, Bi-Ing Chang, Hua-Lin Wu*, Tsung-Lin Cheng*. 2021 Nov. Recombinant thrombomodulin lectin-like domain attenuates porphyromonas gingivalis lipopolysaccharide-induced osteoclastogenesis and periodontal bone resorption. J Periodontol. 92(11):1622-1634.
- Yi-Hsun Huang, Cheng-Hsiang Kuo, I-Chen Peng, Yi-Sheng Chang, Sung-Huei Tseng, Edward M Conway, Hua-Lin Wu*. 2021 Dec. Recombinant Thrombomodulin Domain 1 Rescues Pathological Angiogenesis by Inhibition of HIF-1α-VEGF Pathway. Cell Mol Life Sci. 78(23):7681-7692.
- Cheng-Hsiang Kuo, Ban-Hua Zhang, Shang-En Huang, Jong-Hau Hsu, Yan-Hsiung Wang, Thi Tuyet Ngan Nguyen, Chao-Han Lai, Jwu-Lai Yeh*. 2022 Apr. Xanthine Derivative KMUP-1 Attenuates Experimental Periodontitis by Reducing Osteoclast Differentiation and Inflammation. Front. Pharmacol. 13:821492.
- Yu-Chia Hsu, Hao-Chun Chuang, Kun-Ling Tsai, Ting-Yuan Tu, Yan-Jye Shyong, Cheng-Hsiang Kuo, Yuan-Fu Liu, Shu-Shien Shih, Cheng-Li Lin*. 2022 Oct. Administration of N-Acetylcysteine to Regress the Fibrogenic and Proinflammatory Effects of Oxidative Stress in Hypertrophic Ligamentum Flavum Cells. Oxidative Med Cell Longev. 2022:1380353.
- Cheng-Hsiang Kuo*, Ya-Fang Wu, Bi-Ing Chang, Chao-Kai Hsu, Chao-Han Lai, Hua-Lin Wu*. 2022 Nov. Interference in melanoma CD248 function reduces vascular mimicry and metastasis. J Biomed Sci. 29(1):98.
- Pei-Tzu Yen, Shang-En Huang, Jong-Hau Hsu, Cheng-Hsiang Kuo, Yu-Ying Chao, Lung-Shuo Wang, Jwu-Lai Yeh*.2022 Nov. Anti-Inflammatory and Antioxidative Effects of Puerarin in Postmenopausal Cardioprotection: Roles of Akt and Heme Oxygenase-1. Am J Chinese Med. 51(1):149-168.
- Thi Kim Ngan Ngo, Cheng-Hsiang Kuo, Ting-Yuan Tu*. 2023 Jan. Recent advances in microfluidic-based cancer immunotherapy-on-a-chip strategies. Biomicrofluidics. 17(1):011501.
- Jwu-Lai Yeh, Cheng-Hsiang Kuo, Po-Wen Shih, Jong-Hau Hsu, Peng I-Chen, Yi-Hsun Huang*. 2023 Sep. Xanthine derivative KMUP-1 ameliorates retinopathy. Biomed Pharmacother. 165:115109.
- Shang-En Huang, Cheng-Hsiang Kuo, Si-Yu Shiao, Chia-Rui Shen, Fang-Tzu Lee, Bi-Ing Chang, Jong-Hau Hsu, Hua-Lin Wu, Jwu-Lai Yeh, Chao-Han Lai*. 2023 Jul. Soluble CD93 lectin-like domain sequesters HMGB1 to ameliorate inflammatory diseases. Theranostics. 13(12)4059-4078.
- Chian-Wei Chen#, Chun-Ping Chang#, Yi-Shuan Wen, Cheng-Hsiang Kuo, Shang-Wen Lin, Jui-Chen Tsai, Yan-Jye Shyong*.2023 Dec. Pulmonary delivery of remdesivir and dexamethasone encapsulated nanostructured lipid carriers for enhanced inflammatory suppression in lung. J Drug Deliv Sci Technol. 90:105144.
- Patricia O Benedet, Nooshin S Safikhan, Maria J Pereira, Bryan M Lum, José Diego Botezelli, Cheng-Hsiang Kuo, Hua-Lin Wu, Barbara P Craddock, W Todd Miller, Jan W Eriksson, Jessica T Y Yue, Edward M Conway*. 2024 Jan. CD248 promotes insulin resistance by binding to the insulin receptor and dampening its insulin-induced autophosphorylation. EBioMedicine.99:104906.
- Cheng-Hsiang Kuo*, Gang-Hui Lee, Hua-Lin Wu, Jyun-Yuan Huang, Ming-Jer Tang*. 2024 Feb. Breaking the symmetry of cell contractility drives tubulogenesis via CXCL1 polarization. Proc. Natl. Acad. Sci. U. S. A., 121(9):e2315894121.
- Cheng-Li Lin, Yi-Lian Li, Yu-Wei Chen, Cheng-Hsiang Kuo, Ting-Yuan Tu, Yuan-Fu Liu, Jui-Chen Tsai, Yan-Jye Shyong*.2024 Jun. Amphiphilic NLC-Gel formulation loaded with Sebacoyl dinalbuphine ester and Nalbuphine for localized postoperative pain management. Int. J. Pharm. 659:124295.
- Cheng-Li Lin, Yu-Wen Su, Yu-Wei Chen, Cheng-Hsiang Kuo, Ting-Yuan Tu, Jui-Chen Tsai, Yan-Jye Shyong*. 2024 Jun. BMSC loaded photo-crosslinked hyaluronic acid/collagen hydrogel incorporating FG4592 for enhanced cell proliferation and nucleus pulposus differentiation. Int. J. Biol. Macromol. 273(Pt 1):132828.
- Ya-Chu Ku, Yao-Chou Lee, Yi-Kai Hong, Yung-Ling Lo, Cheng-Hsiang Kuo, Kuan-Chieh Wang, Chao-Kai Hsu, Chien-Hung Yu, Shu-Wha Lin*, Hua-Lin Wu*. 2024 Sep. Deciphering the dysregulating IGF-1-SP1-CD248 pathway in fibroblast functionality during diabetic wound healing. J Invest Dermatol. In press doi: 10.1016/j.jid.2024.07.035.
- Cheng-Li Lin, Yu-Wei Chen, Cheng-Hsiang Kuo, Ting-Yuan Tu, Hua-Lin Wu, Jui-Chen Tsai, Yan-Jye Shyong*. 2024 Sep. Calcium phosphate complex of recombinant human thrombomodulin promote bone formation in interbody fusion. Biofabrication. In press doi:10.1088/1758-5090/ad8035
Conference posters or talk
- Cheng-Hsiang Kuo, Po-Ku Chen, Hua-Lin Wu, Guey-Yueh Shi*. 2011 Jul. The role of thrombomodulin and Lewis Y antigen on vascular endothelial tube formation. XXIII Congress of the International Society on Thrombosis and Haemostasis. ISTH2011. Kyoto, Japan.
- Cheng-Hsiang Kuo, Yen-Ju Chen, Guohao Dai, Yun Fang*. 2014 Apr. Mechanosensitive lipid phosphate phosphatase 3 (PPAP2B) regulates athero-relevant endothelial phenotypes. 6th Annual Morton Arnsdorf Cardiovascular Research Day Symposium. Chicago, USA.
- Lan-Yun Chang, Cheng-Hsiang Kuo, Hua-Lin Wu, Chao-Han Lai* 2020 Dec. Recombinant thrombomodulin lectin-like domain attenuates osteoclastogenesis and periodontal bone resorption. 2020 International Symposium of Materials on Regenerative Medicine. Tainan, Taiwan
- Ru-Han Sie, Cheng-Hsiang Kuo, Hua-Lin Wu* 2021 Nov. Thrombomodulin promotes melanoma vascular mimicry through focal adhesion kinase-dependent pathway. 2021 International Conference on Advanced Biomedical Sciences. Taichung, Taiwan
- Thi Kim Ngan Ngo, Hua-Lin Wu, Cheng-Hsiang Kuo* and Ting-Yuan Tu* 2022 Jun. Investigating the effect of thrombomodulin on melanoma cancer metastasis using three-dimensional tumor spheroid model. 9th World Congress of Biomechanics. Taipei, Taiwan
- Cheng-Hsiang Kuo* 2023 Jan. Modeling renal tubulogenesis in a dish – focusing on the mechanoregulation. 2023 Taiwanese Physical Society Satellite Meeting: Emerging Behaviors of Collective Cells. Tainan, Taiwan. (invited talk)
- Ru-Han Sie, Ya-Fang Wu, Bi-Ing Chang, Cheng-Hsiang Kuo*, Hua-Lin Wu* 2023 Mar. Melanoma CD248 promotes vascular mimicry and metastasis. The 37th Joint Annual Conference of Biomedical Science. Taipei, Taiwan.
- Thi Kim Ngan Ngo, Hua-Lin Wu, Cheng-Hsiang Kuo*, Ting-Yuan Tu* 2023 Apr. Effect of thrombomodulin and plasminogen interaction on melanoma spheroid invasion. 2023 American Association for Cancer Research Annual Meeting. Orlando, USA.
- Cheng-Hsiang Kuo*, Gang-Hui Lee, Hua-Lin Wu, Jyun-Yuan Huang, Ming-Jer Tang* 2024 Mar. Breaking the symmetry of cell contractility drives tubulogenesis via CXCL1 polarization. The 38th Joint Annual Conference of Biomedical Science. Taipei, Taiwan.
- Cheng-Hsiang Kuo* 2024 Nov. Mechanoregulation of tubulogenesis in renal proximal tubular epithelial cells. The 5th International Symposium on Mechanobiology. Tainan, Taiwan. (invited talk)
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